This product uses a chip as a carrier for nucleic acid extraction and qPCR amplification testing of samples. It is characterized by simple operation, easy to use, and low laboratory requirements.

It can be used to detect seven common pathogens including EHP, SHIV, EMS, WSSV, IHHNV, VP, and VH in shrimp larvae, adult shrimp, water, and feed. The lowest detection limit is 1000 copies/ml of the pathogen.

The matching product is our Virus Hunter Plus series instrument.

Related products include nucleic acid detection reagent kits for aquatic diseases and the COVID-19 N&O gene detection chip kit.

This project platform is developed based on the Virus Hunter Plus device and LAMP-customized microfluidic chip platform, and develops a reagent kit that can quickly and multiply detect animal-sourced nucleic acid components in food and meat products on a small chip.

The "Animal-sourced Six-item Nucleic Acid Detection Chip Kit" developed by this project can detect meat-sourced components including chicken, duck, goose, pork, beef, and lamb, and can achieve a detection limit of 1%. The positive and negative compliance rates of clinical samples are above 95%, with high specificity and stability and other performance indicators.

This product is equipped with the "Animal-sourced 6-item Component Nucleic Acid Detection Chip Kit Operation Full Process Manual", which provides detailed instructions from sample preparation, nucleic acid extraction to microfluidic chip and equipment operation.

This product includes animal-sourced six-item nucleic acid detection chips, positive control products (six-item positive mixed plasmids), negative control products (ultrapure water or TE buffer), white oil, reaction solution (MIX), enzymes, and product instructions.

Currently, the nucleic acid detection methods used for animal-sourced food adulteration on the market are complex and have low detection efficiency. The rapid and multiply detection products developed by this project platform have the advantages of rapid and multiply detection, which can solve the current low efficiency and complex operation of nucleic acid detection methods on the market.

This product is based on the real-time fluorescence quantitative PCR method using one-step reverse transcription method and TaqMan fluorescence probe technology. It is manufactured using the full-component glass state process and can be used to add a larger volume of nucleic acid template, resulting in higher detection sensitivity. It can amplify specific nucleic acid fragments of throat swabs and determine the results by real-time amplification curves. This product is used to detect the N gene and ORF1ab gene of nCOV-19, and FluA and FluB of influenza A and B, with RNase P as the internal reference. The detection limit of the chip is 1000 copies/mL, with a coefficient of variation less than 5%.

Component name Specification

Up Respiratory Tract Virus 3-item (nCOV-19/FluA/FluB) Nucleic Acid Detection Chip 5 pieces/box

Up Respiratory Tract Virus Positive Control - Pseudo Virus 1 .1 mL × 1 tube

Up Respiratory Tract Virus Negative Control 1 .1 mL × 1 tube

Virus preservation solution 10 mL × 1 tube

Swab 5 pieces

User Manual 1 piece

This project is based on the Virus Hunter Plus platform, adapted to the microfluidic chip platform, and provides a method for detecting 7 pathogens of cat respiratory and zoonotic diseases. It has the advantages of fully automatic integration and can achieve fast and accurate simultaneous detection of multiple pathogens, including feline calicivirus FCV, feline herpesvirus FHV, feline Mycoplasma MYF, feline Chlamydia MYC, feline bronchial septic Pasteurella FBb, Toxoplasma gondii (TG), and Leptospira interrogans (Lep) (including cat reference genes). It is of great significance for the clinical diagnosis and treatment of cat respiratory and zoonotic diseases.